Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: Transcriptomic data suggest that Th17 cell/IL-17A-related signaling is involved in the Pasteurella multocida serotype A infection. A Principal component analysis (PCA) of the transcriptomic data of the PmCQ2 groups and Con groups. B Transcriptomic analysis of significantly different genes between the PmCQ2 groups and Con groups. C Gene Ontology (GO) analysis of PmCQ2 groups versus Con groups and the top 30 GO terms are shown. D Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of PmCQ2 groups versus Con groups; the top 20 pathways are listed. E Th17 cell differentiation-associated genes identified via KEGG are shown. Red indicates upregulation and blue indicates downregulation. F Transcription levels of IL-6, IL-23, and TGF-β in PmCQ2-infected murine lungs at 24 hpi. G Representative images of mIHC staining of IL-17A and CD4 in Con- and PmCQ2-infected murine lungs at 32 hpi. The double-positive cells were mature Th17 cells. Scale bar = 100 μm. H Flow cytometry analysis of Th17 cells in PmCQ2-infected murine lungs at 32 hpi. (Right) quantification of Th17 cells in PmCQ2-infected murine lungs at 32 hpi. I Transcription levels of IL-17A and IL-22 in PmCQ2-infected murine lungs at 24 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Cell Differentiation, Staining, Flow Cytometry

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: Th17 cells inhibit Pasteurella multocida serotype A infection. A Representative images of mIHC staining of the macrophage marker CD68 and the T-cell marker CD3 in Con- and PmCQ2-infected murine lungs at 32 hpi. Scale bar = 100 μm. B Flow cytometry analysis and quantification of CD3 + T cells in Con- and PmCQ2-infected murine lungs at 32 hpi. C A scheme showing the GSK805 (30 mg/kg) treatment protocol at the top. The survival curves of PmCQ2-infected mice treated with or without 30 mg/kg GSK805 are shown at the bottom. D A scheme presents the in vitro Th17 cell differentiation and treatment assay protocol. E Survival curves of PmCQ2-infected mice treated with or without 10 6 Th17 cells. F Representative photographs of murine lungs infected with PmCQ2 plus saline or Th17 cells at 32 hpi. G Representative images of HE staining of murine lungs infected with PmCQ2 plus saline or Th17 cells at 32 hpi. Scale bar = 200 μm. H The bacterial load of the infected lungs and blood at 32 hpi. I Quantification of IL-6, TNF-α, and IL-1β PmCQ2-infected murine serum at 32 hpi. J Quantification of AST, ALT, BUN, and CREA levels in PmCQ2-infected murine serum at 32 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Staining, Marker, Flow Cytometry, In Vitro, Cell Differentiation, Saline

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: IL-17A, a Th17 cell effector, restricts Pasteurella multocida serotype A infection. A Quantification of IL-17A in PmCQ2-infected murine blood and lungs at 32 hpi. B Western blot analysis and quantification of IL-17A in PmCQ2-infected murine lungs. C A schematic of the mouse IL-17A neutralizing antibody treatment protocol. D Survival curves of PmCQ2-infected mice treated with or without 200 μg of mouse IL-17A neutralizing antibody. E The bacterial load of the infected lungs and blood at 32 hpi. F Representative photographs of murine lungs infected with PmCQ2 plus IgG or 200 μg of mouse IL-17A neutralizing antibody at 32 hpi. G Representative images of HE staining of murine lungs infected with PmCQ2 plus IgG or 200 μg of mouse IL-17A neutralizing antibody at 32 hpi. Scale bar = 200 μm. H Quantification of IL-6, TNF-α, and IL-1β PmCQ2-infected murine serum at 32 hpi. I Quantification of AST, ALT, BUN, and CREA levels in PmCQ2-infected murine serum at 32 hpi. J A schematic of the 10 μg/kg recombinant mouse IL-17A treatment protocol. K Survival curves of PmCQ2-infected mice treated with or without 10 μg/kg recombinant mouse IL-17A. L Representative photographs of murine lungs infected with PmCQ2 plus 10 μg/kg BSA or 10 μg/kg recombinant mouse IL-17A at 32 hpi. M Representative images of HE staining of murine lungs infected with PmCQ2 plus 10 μg/kg BSA or 10 μg/kg recombinant mouse IL-17A at 32 hpi. Scale bar = 200 μm. N The bacterial load of the infected lungs and blood at 32 hpi. O Quantification of IL-6, TNF-α, and IL-1β PmCQ2-infected murine serum at 32 hpi. P Quantification of AST, ALT, BUN, and CREA levels in PmCQ2-infected murine serum at 32 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Western Blot, Staining, Recombinant

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: STAT3 is critical for Th17 cell development during Pasteurella multocida serotype A infection. A Transcription levels of Stat3 in PmCQ2-infected murine lungs at 24 hpi. B Western blot analysis and quantification of p-Stat3 and Stat3 in PmCQ2-infected murine lungs. C A scheme presents the Stattic treatment assay protocol. D Western blot analysis and quantification of p-Stat3 and Stat3 in PmCQ2-infected murine lungs. E Survival curves of PmCQ2-infected mice treated with or without 15 mg/kg Stattic. F The bacterial load of the infected lungs and blood at 32 hpi. G Photographs of murine lungs infected with PmCQ2 plus vehicle or 15 mg/kg Stattic at 32 hpi. H Representative images of HE staining of murine lungs infected with PmCQ2 plus vehicle or 15 mg/kg Stattic at 32 hpi. Scale bar = 200 μm. I Quantification of IL-6, TNF-α, and IL-1β PmCQ2-infected murine serum at 32 hpi. J Quantification of AST, ALT, BUN, and CREA levels in PmCQ2-infected murine serum at 32 hpi. K Western blot analysis and quantification of IL-17A in PmCQ2-infected murine lungs. L Representative images of mIHC staining of IL-17A and CD4 in Con- and PmCQ2-infected murine lungs at 32 hpi. The double-positive cells are mature Th17 cells. Scale bar = 100 μm. M Flow cytometry analysis and quantification of Th17 PmCQ2-infected murine lungs at 32 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Western Blot, Staining, Flow Cytometry

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: Regulation of Th17 cell/IL-17A activation via the IL-6–JAK2–STAT3 axis in Pasteurella multocida serotype A infection. A Transcription levels of IL-6, Jak2, and Stat3 in PmCQ2-infected murine lungs at 24 hpi. B Quantification of IL-6 in PmCQ2-infected murine serum in a time-dependent manner. C Western blot analysis and quantification of p-Jak2, Jak2, p-Stat3, and Stat3 in PmCQ2-infected murine lungs in a time-dependent manner. D A schematic of the IL-6-KO mouse treatment protocol at the top. The survival curves of PmCQ2-infected IL-6-KO mice treated with or without 10 μg/kg recombinant mouse IL-17A are shown. E Western blot analysis and quantification of p-Jak2, Jak2, p-Stat3, and Stat3 in PmCQ2-infected murine lungs. F Representative images of mIHC staining of IL-17A and CD4 in Con- and PmCQ2-infected murine lungs at 32 hpi. The double-positive cells are mature Th17 cells. Scale bar = 100 μm. G Flow cytometry analysis and quantification of Th17 PmCQ2-infected murine lungs at 32 hpi. Every point represents one individual. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Activation Assay, Infection, Western Blot, Recombinant, Staining, Flow Cytometry

Journal: Veterinary Research

Article Title: Th17 cells/IL-17A shape Pasteurella multocida serotype A infection in murine and rabbit models

doi: 10.1186/s13567-025-01662-1

Figure Lengend Snippet: Schematic showing that Th17 cells/IL-17A shape Pasteurella multocida serotype A infection. Serotype A Pasteurella multocida (PmA) causes systemic infection and excessive release of inflammatory factors by destroying the lung barrier. In contrast to previous reports that PmA induces an excessive immune response, although PmA can induce a Th17 cell response, it inhibits T-cell immunity, resulting in insufficient activation of Th17 cells and their effector molecule IL-17A, which ultimately limits the clearance of PmA. At the molecular level, the IL-6–JAK2–STAT3 axis is strongly involved in Th17 cell/IL-17A activation during PmA infection. Importantly, targeting Th17 cells and IL-17A has potential for clinical application, as evidenced by the significant attenuation of PmA-induced lung injury and systemic inflammation as well as the reduction in animal mortality.

Article Snippet: Th17 cells were identified via a Mouse Th17 Cell Flow Cytometry Staining Kit (Elabscience, XJM002).

Techniques: Infection, Activation Assay